|
KMID : 1041219980400020126
|
|
Journal of Sericultural and Entomological Science
1998 Volume.40 No. 2 p.126 ~ p.130
|
|
|
Comparison of Characteristics between Insecticidal and Noninsecticidal Bacillus thuringiensis Strains belonging to Serotype H8a8b
|
|
Roh, Jong Yul
Je, Yeon Ho/Park, Hyun Woo/Chang, Jin Hee/Jin, Byung Rae/Lee, Dae Weon/Ziwen Yang
|
|
|
Abstract
|
|
|
|
A noninsecticidal strain, Bacillus thuringiensis NTB-88, isolated from Korean soil, had a typical bipyramidal parasporal inclusion and its serotype is identical to B. thuringiensis subsp. Morrisoni(H8a8b). To elucidate differences between insecticidal and noninsecticidal strains, we compared strain NTB-88 to other toxic B. thuringiensis subsp. morrisoni strains (HD-12 and PG-14). Restriction endonucleases digested plasmid DNA patterns showed that strain NTB-88 was different from lepidopteran-toxic strain, HD-12, but it was similar to dipteran-toxic strain, PG-14. The gene type of strain NTB-88 was different from those of other insecticidal strains. Furthermore, the NH2-terminal amino acid sequence of crystal protein of strain NTB-88 had no relation to those of the previously known δ-endotoxins in other toxic strains as well as HD-l2 and PG-14 strains. Therefore, the noninsecticidal crystal protein in strain NTB-88 is novel and its property is different from insecticidal ones.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|